![]() There is some repair processes going in, cell is re-synthesizing damaged cell constituents in preparation for binary fission. The cells are also adapting and adjusting to this media and growth condition, different genes may be turned on to start metabolizing different substrates. Although there is no apparent cell division occurring, the cells may be growing in volume or mass, synthesizing enzymes, proteins, RNA, etc., and increasing in metabolic activity. Lag Phase: immediately after inoculation of the cells into fresh medium, the population remains temporarily unchanged (notice the line here is flat, no change in cell number). The phases are lag, log/exponential, stationary, and death phases. There are typically four growth phases in a closed bacterial culture vessel, like a flask or tube. The curve can then be used to determine the generation time, the time required for a microbial population to double in cell number. ![]() This growth curve is generated by plotting the increase of cell number versus time. The dynamics of bacterial growth follow a predictable pattern visualized as a bacterial growth curve. Watch Video 1: Bacterial cell division (00:15).
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